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New Antibiotics and Biopreservatives To Substitute Imported Counterparts

Currently, the Russian market is dominated by the US and Chinese feed antibiotics and biopreservatives (70% of sales). The task of the development of import-substituting products for livestock production technologies using the available domestic raw materials is solved by participants of the complex project, which is supported by the Federal Target Program “Research and development on priority directions of scientific and technological development of Russia for 2014-2020.”

Altai State University, Altai State Agrarian University, small innovative enterprise “INZHBIO” LLC and “Fermlab” are among the performers of the project. Industrial partner is “Business Concept” LLC engineering company.

Vakhtang_Javakheti
Vakhtang Javakheti

Technology for producing feed antibiotic is antibiotic biosynthesis by means of fermentation-producing strain, isolation and chemical purification of the antibiotic followed by the manufacture of the formulation of a biological product. “The problem nowadays is the low productivity-producing strains.

Our task is to develop a highly antibiotic-producing strain and production technology for efficiency in excess of 10-15% figures from foreign manufacturers.

In the bulk manufacture the difference of 10-15% is very significant,” - the head of the Laboratory of Biotechnology of physiologically active substances of the Federal Research Center “Biotechnology” of RAS, Vakhtang Javakheti, reported. The problem is solved, according to him, by means of non-directional multi-mutagenesis-producing strain followed by selection, the development of efficient technologies biosynthesis, separation and chemical purification of the antibiotic.

The problem in the application of biopreservatives is a consortium of bacteria in canning crops that are hard to ensilage, growing on inaccessible sugars, which drastically reduces its effectiveness. “It is necessary to add to the preparation the required enzyme hydrolyzing polysaccharides into simple sugars. We use the method of identification of target genes in related strains hydrolyzing polysaccharides as a result of genome sequencing and create a bioengineered strain, which is part of the consortium expressing the gene glycoside hydrolase (xylanase)”, - Vakhtang Javakheti said.

To date, we developed a working design documentation for a pilot plant producing feed antibiotics, working design documentation for a pilot plant producing of a biopreservative pilot-scale production technology of feed antibiotics and a biopreservative in lyophilized form. Besides, pilot batches of these medicines for the registration tests were manufactured and technological regulations of feeding farm animals were developed. The methods of identification of the residual content of antibiotics in animal tissues after use were developed.

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The state registration of feed antibiotics and biopreservative, working hours and the placing on the market of industrial batches are expected in 2018. “As a result, livestock farms have access to feed antibiotics and biopreservatives of Russian production to replace imported analogues. This will achieve 35-40% savings on purchases of foreign products, improve the efficiency of livestock farming base, and thus the food sovereignty of the country,” - the Head of the Laboratory of Biotechnology of physiologically active substances of the Federal Research Center “Biotechnology” of RAS summed up.

Another breakthrough of Russian scientists has recently been made by the international team of scientists, who managed to find out that vitamin B2 (riboflavin) can be used to treat cancer. The authors found a way to activate riboflavin tissue, which leads to death of cancer cells. The study is published in top journal Scientific Reports. The work was supported by grants from the Russian Science Foundation (RSF).

“The problem was that previously riboflavin couldn’t be activated in cancer. We found a way to photoactivate riboflavin in the depths of biological tissue through the use of the so-called apconverting nanoparticles”, - one of the authors of the article, the head of RSF grant, the candidate of Physical and Mathematical Sciences, Yevgeny Khaidukov, says.

Previously it was known that a complex cascade of chemical reactions can result in production of riboflavin reactive oxygen species which destroy the cancerous tumor. But the crux of the matter was to ensure, to activate (excite) riboflavin and start a cascade of reactions. The fact that riboflavin comes to an excited state when it is affected by the light rays in the blue or ultraviolet (UV) range. However, such a light doesn’t penetrate into the biological tissue to a depth sufficient to destroy the tumor.

That’s what it took to create apconverting nanoparticles. Their peculiarity is that they are able to turn the faint infrared (IR) light that is not harmful to humans but penetrates into the depth of our body tissues, into the ultraviolet.

Scientists managed to establish an active complex containing riboflavin and apconverting nanoparticles that trigger a cascade of reactions leading to the release of reactive oxygen species and, hence, to destruction of cancerous cells. This complex is taken intravenously.

“So far, the studies were conducted on laboratory mice. There were special models to which human cancer was grafted. Single exposure was observed in inhibition of growth of tumor cells of breast adenocarcinoma and reducing its volume by 90%”, - Yevgeny Khaidukov says.

The scientists plan to cooperate further with physicians from N.N. Blokhin RONF, who are also the authors of the submitted work. The next step is clinical trials of the resulting methodology.

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